MESF and ABC values provide standardized units of fluorescence intensity.Precise MESF or ABC values are assigned to bead populations through meticulous primary calibrations.Bangs’ Quantum™ kits are uniquely qualified for applications in quantitative fluorescence cytometry: The MESF or ABC values of labeled cell samples may be determined by measuring their fluorescence intensities, and “reading” the corresponding MESF or ABC values from the standard curve using the QuickCal ® analysis template that is provided with the kit. Fluorochrome-labeled microspheres are used to generate a standard curve relating fluorescence intensity to standardized MESF or ABC values from Quantum™ MESF or Quantum™ Simply Cellular ® beads. Our products include Quantum™ MESF (Molecules of Equivalent Soluble Fluorochrome) and Quantum™ Simply Cellular ® (ABC, Antibody Binding Capacity) kits. Tools for Quantitation Bangs’ tools for quantitative flow cytometry provide the means to standardize fluorescence intensity measurements, thereby permitting truly quantitative analyses. The interpretation of fluorescence intensity measurements can be further complicated by factors such as daily instrument variation, differences in hardware (laser power, filter sets), PMT voltages, software, environmental factors such as buffer pH, and fluorochrome labeling density of antibodies (F/P ratio).
Notably, without a standardized measure of fluorescence intensity, results of analyses can be described only in relative terms, such as negative / positive, dim / intermediate /bright, or in arbitrary fluorescence intensity units. Although fluorescence cytometry has proven to be a very powerful and versatile technology, it is not without limitations. This technology is routinely used for immunophenotyping and an expansive array of research applications, such as the study of protein phosphorylation and the determination of telomere length. Quantitative Cytometry Applications Fluorescence cytometry is an important tool for investigations in cell and molecular biology. 800.387.0672 Tools for Expression Analysis, Standardize measurements to ensure accurate and comparable results.
0 kommentar(er)
